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Graphical Abstract 1 Introduction Glaucoma refers to a heterogeneous group of chronic, progressive optic neuropathies characterized by the irreversible loss of retinal ganglion cells, leading to thinning of the retinal nerve fiber layer and subsequent visual field loss ( These factors contribute to the development of glaucoma-related ocular surface disease (G-OSD), a condition that often goes underdiagnosed but is estimated to affect 40%–59% of glaucoma patients worldwide ( Glutathione (GSH), a low molecular weight thiol-containing compound, is the most abundant biological antioxidant present in mammalian tear fluid ( in vitro or in vivo —has not yet been explored