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GST or GST-VIME expressed in Escherichia coli BL21 were incubated with Flag-MAGE-G1 expressed in HEK293T, and precipitated by Glutathione-Sepharose 4B beads
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Interestingly, wild-type p53 interacts with DNA (cytosine-5)-methyltransferase (DNMT1) to increase the de novo DNA methylation and gene silencing capacity of DNMT1, suggesting functional p53 may use DNMT1 and epigenetic plasticity to evade apoptosis in low methionine stress conditions [53]
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