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Consistent with this notion, iscX was found to have a weaker genetic link with cyaY than with other genes of the isc operon among Proteobacteria

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For co-localization experiments, cells were further incubated with anti-EGFR (Cell Signaling Technology TM, 2232S) (1:500) for 1 h at room temperature (or overnight at 4°C), washed twice with 0.1% Tween 20 in PBS, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:200) for 1 h, followed by washing again