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The role of IL-17A in promoting the development of intestinal tumors has been extensively studied, and the removal of IL-17A has a significant impact in reducing the number of tumors in ApcMin mice (238, 239)
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Proteins were then precipitated by addition of 500 µl methanol (pre-cooled at –80 °C), 400 µl LCMS-grade water, and 100 µl chloroform
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