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To assess phosphorylation in vitro, substrates GST-G6PD WT, GST-2YF or GST-2YA (1.5 µg) was incubated with recombinant SRC (0.5 µg) for 30 min at 37 °C in a 50 μl kinase reaction system (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 100 mM DTT, 10 mM MgCl 2 ) with 0.3 mM ATP
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