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Key Takeaways G-protein-coupled receptors rely on the activation of G-proteins to cause cellular changes G-protein-coupled receptors have slower effects than ligand-gated receptors G-proteins can open ion channels, alter protein function via phosphorylation, and alter gene transcription The G s subunit initiates the adenylyl cyclase / cAMP signaling pathway The G i subunit inhibits the adenylyl cyclase / cAMP signaling pathway The G q subunit initiates the phospholipase C / IP 3 / DAG signaling pathway Test Yourself