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Glutathione Wellness Clinics: What to Know Before You Go | InCare

After blocking in 5% milk, membranes were placed in antibodies against SOD1 (Santa Cruz Biotechnology, Dallas, TX, USA, #sc-515404 C-8, working concentration 1:1000), SOD2 (Merck Sigma-Aldrich, Darmstadt, Germany, #06-984, 1:1000), Actin (Santa Cruz Biotechnology, Dallas, TX, USA, #sc-1616, 1:500), Actin (Abcam Limited, Cambridge, UK, #ab6276, 1:10000), TSC2 (Cell Signaling, Cambridge, UK, #43085, 1:2000), S6RP (Cell Signaling, #2217 s, 1:1000), P-S6RP (Ser 235/236, Cell Signaling, #4858 s, 1:2000), P-S6RP (Ser 240/244, Cell Signaling, #5364, 1:1000), 4-EB-P1 (Cell Signaling, #9452, 1:1000), P-4E-BP1 (Ser 65, Cell Signaling, #2855, 1:1000) overnight

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