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Results and discussion Principle of [GSH]:[GSSG] determination in the ER With the aim to monitor the [GSH]:[GSSG] ratio in the ER of live cells, we fused the C30S mutant of yeast glutaredoxin 1 (sCGrx1p) with N-terminal signal peptide and HA-tag and C-terminal KDEL ER retrieval motif (Fig
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