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Total proteins were incubated in 30 µL of kinase buffer (20 mM Tris–HCl, pH 8.0, 1 mM DTT, 5 mM MgCl 2 , and 10 μM ATP) at 30 °C for 30 min, His-tagged PKS5 were removed by a 5-min centrifugation at 500 × g at 4 °C
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[DOI] [PMC free article] [PubMed] [Google Scholar] 53.Harrison S.A., Baum S.J., Gunn N.T., Younes Z.H., Kohli A., Patil R., Koziel M.J., Chera H., Zhao J., Chakravarthy M.V
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