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Although physical activity associated with sports performance will be covered in detail in the following sections, it is necessary to mention that the formation of ROS during skeletal muscle contraction and physical activity has been the subject of intense discussion for the past four decades and still is
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Reactions were initiated by the addition of 10× reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl₂, 50 mM ATP, 5 mM DTT) and incubated at 37 °C for the specified durations
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