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RNA isolation and quantitative real-time RT-PCR Total RNA was isolated from Jurkat T cell using Trizol (Invitrogen, Carlsbad, CA, USA)

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Type:Vitamin D3 + K2

Example 4: dissolving 0.20g of copper hydroxide in 20ml of distilled water, adding 0.68g of GHK tripeptide under the condition of stirring, adding 0.12ml of glacial acetic acid, reacting for 24 hours under the condition of stirring at 40 ℃, filtering, concentrating and freeze-drying to obtain 0.575g of GHK copper peptide complex with the purity of 99%, the yield is 72%, the acetic acid content is 15.9%, and the copper content is 7.1%