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2.4 Isolation of liver mitochondria Mitochondria were obtained using methods described in the literature referred to 2.5 Evaluation of MPT Liver mitochondria were isolated and resuspended (0.25 mg of protein/mL) in an incubation medium (250 mM sucrose, 1 mM P i -Tris, 10 mM Tris-MOPS, 5 mM glutamate-Tris, 2.5 mM malate-Tris, pH 7.4, 25°C)

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(PubMed) Walker JG, Mackinnon AJ, Batterham P, et al