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After viability evaluation by exclusion of trypan blue dye, monocytes were seeded at 1.25 x 10 6 cells/mL in R10 medium (RPMI medium with 10% FBS and 1% penicillin) and cultivated in a cell culture flask at 37°C in a humidified atmosphere containing 5% CO 2 for 4 days in the presence of IL-4 and GM-CSF (both at a concentration of 12.5 ng/mL, R&D Systems) as previously described ( Figures 1C, D

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