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In human GGT, autocatalytic activation involves an N-O acyl shift at threonine (Thr)-381, which produces an ester bond between the side chain OH group of the Thr and an α-carbonyl group of the residue 380, which is the last residue of the N-terminal portion that consequently becomes the large subunit
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Consider whether an acceptable alternative could meet the same purpose. Avoid allowing a short deadline to erase an essential requirement.
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