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FIGURE 5 The indirect effect of PBDE and the role of the placenta in regulating FB neuroinflammation Next, to evaluate the effect of PDBE exposure within the biological context, PDBE-conditioned media collected from the fetal outlet chamber of the PLA-OOC, containing PDBE metabolites and PBDE from the PLA-OOC, was introduced to HBMEC, and treatment completed for 72 h (Figure 4A–FB-OOC Experiment)
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As expected, GSH level was 2 times lower in FlpIn 293 expressing MRP1 (30 ± 5 nmol/mg protein) than that of the control cells transfected with an empty vector (55 ± 5 nmol/mg protein, taken as 100% intracellular GSH) reflecting the basal GSH transport by MRP1
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