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Immediately after fixation the samples were washed once briefly with MilliQ and stained overnight at 37°C with fresh SA-β-GAL solution (pH 6.0), containing 2,5 mM Na2HPO4, 7,4 mM Citric Acid, 0.15M NaCl, 2mM MgCl2, 5 mM Potassium Ferricyanide, 5 mM Potassium Ferrocyanide and 25ul/ml 4% X-gal in DMF