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The deficiency of micronutrients exacerbates insulin resistance, oxidative stress and hormonal dysregulation through their negative impact on PI3K/Akt, NF-κB and Nrf2 and steroidogenic enzyme signaling pathways, all of which play a key role in the pathophysiology of PCOS
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Future work should pair super-resolution and functional imaging with organelle-addressed reporters, localized perturbations (isoform-specific NOX/NOS, ER oxidoreductases, RET modulators) and computational models that capture vectorial flow rather than free diffusion
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