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Materials and methods Materials Aluminium foil (Al, 99.998%, 0.5 mm thick, Alfa Aesar, Ward Hill, MA, USA), acetone (C3H6O, 99.3%, Piochem, Giza, Egypt), sodium hydroxide (NaOH, 98–99%, 39.997 g/mol, El Nasr pharmaceutical chemicals company, Cairo, Egypt), absolute ethanol (CH 3 CH 2 OH, 99.9%, 46.07 g/mol, Merck, Darmstadt, Germany), perchloric acid (HClO 4 , 71–73%, Advent, Mumbai, India), oxalic acid dihydrate (H 2 C 2 O 4 ·2H 2 O, 99.82%, 126.07 g/mol, Advent, Mumbai, India), chromium trioxide extra pure (CrO 3 , 99%, 99.99 g/mol, Laboratory Rasayan, Gujarat, India), orthophosphoric acid (H 3 PO 4 , 85%, El Nasr pharmaceutical chemicals company, Cairo, Egypt), sulphuric acid (H 2 SO 4 , 95–97%, Merck, Darmstadt, Germany), copper (II) chloride (CuCl 2 , 99%, 134.45 g/mol, Advent, Mumbai, India), hydrochloric acid (HCl, 37%, El Nasr pharmaceutical chemicals company, Cairo, Egypt), polymethyl methacrylate (PMMA) ( [CH 2 C(CH 3 )(CO 2 CH 3 )] n , Mw ~ 15,000, Sigma Aldrich, Darmstadt, Germany), polyurethane (PU) (Sigma Aldrich, St

SwiftSupps

Count:30ct

Therefore, there is an attempt to induce methyl deficiency through feed additives, such as guanidinoacetic acid (GAA), to better evaluate the role of RPM in supporting methyl donor supply

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