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For both techniques, the supernatant of Vero E6 cells was removed and the cells were inoculated with 50 μl/well of sera-virus mixture and incubated for 1 h at room temperature under gentle shaking, allowing the infection of cells by non-neutralized viral particles

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These findings can be correlated with post-infection fatigue among covid19 patients, as covid-associated immune response also leads to the systemic production of cytokine thereby, leading to fatigue and fibromyalgia [47]