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After blocking with 5% skimmed milk, the membranes were incubated with antibodies against the following proteins: iNOS (BD Biosciences, San Jose, CA, USA), CD80, CD86, PI3K, p-PI3K (Tyr458), p70S6K, p-p70S6K (T389), STING, p-STING (Ser365), p65, p-p65 (Ser536), ATF4, IRF3, p-IRF3 (Ser396), IKKβ, p-IKKα/β (T176/180), IκBα, p-IκBα (S32), p-IκBα (S32/36) , p-TAK1 (T184/187), AKT, p-AKT (Ser473), mTOR, MyD88, TBK1 (Cell Signaling Technology, Danvers, MA, USA), TAK1 (ABclonal Technology, Boston, MA, USA), and β-Actin (Sigma)
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91 Deferoxamine (DFO), as an iron chelator to remove iron from the liver, exhibited significant improvement in bone mineralization alongside its significant effect on liver function test in a rat model of liver cirrhosis-induced osteoporosis
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