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Likewise, 527 bp of the upstream and 446 bp of the downstream regions of the GDH3 gene were amplified and fused with the SAT1 fragment to form a disruption cassette (~6 kb) for GDH3 gene deletion (Supplementary Fig

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Prior to this study, only intravenous administration of amino acids was shown to reliably increase endogenous hGH 14, 15 while studies of the effects of oral administration of amino acids on hGH release yielded conflicting results