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A thrombin recognition site and linker sequence (GLVPRGSAAAA) were inserted between GluK3 and the coding sequence for the A207K non-dimerizing EGFP mutant, with a C-terminal octa-histidine (His8) affinity tag, in order to screen constructs via fluorescence detection and for affinity purification
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In general, tryptophan metabolism is also impaired in IBD
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Consider whether an acceptable alternative could meet the same purpose. Avoid allowing a short deadline to erase an essential requirement.
Use a cropped view when it fully supports your intended point. Keep enough context to avoid misrepresenting the information shown.
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