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Such modifications may damage mtDNA and impair mitochondrial function, while older individuals who experience prolonged stress are more likely to experience multiple molecular changes, such as the release of endogenous neurotoxins, higher oxidative stress levels, and epigenetic modifications

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Subcutaneous xenograft model To establish the GC xenograft tumor model, 1 × 10 7 NUGC-4 GC cells in 100 µL of PBS mixed with 100 µL of Matrigel (BD Biosciences) were injected subcutaneously into the right flanks of four-week-old male nude mice