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The TNF-α-induced decrease in TER is well correlated with MLCK expression and MLC phosphorylation.42 In addition, in intestinal HT-29/B6 cells, TNF-α was seen to decrease TER through claudin-2 expression.43 Claudin-2 is expressed throughout the intestine and forms a pore for cations, which is blocked by involvement of phosphatidyl inositol-3 kinase (PI3K)/Akt signaling
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